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hiscript iii reverse transcriptase kit  (Vazyme Biotech Co)


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    Structured Review

    Vazyme Biotech Co hiscript iii reverse transcriptase kit
    Hiscript Iii Reverse Transcriptase Kit, supplied by Vazyme Biotech Co, used in various techniques. Bioz Stars score: 96/100, based on 717 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/hiscript+iii+reverse+transcriptase/Hiscript+III+Reverse+Transcriptase/pm41879300-227-20-25
    Average 96 stars, based on 717 article reviews
    hiscript iii reverse transcriptase kit - by Bioz Stars, 2026-09
    96/100 stars

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    Related Articles

    Reverse Transcription:

    Article Title: Combating VEGFA-siRNA-Induced Metabolic Reprogramming via Glucose Utilization Deprivation.
    Article Snippet: YeaRed Nucleic Acid Gel Stain (10202ES76), Annexin V-FITC/PI Apoptosis Assay Kit (40302ES60), TUNEL Apoptosis Detection Kit (FITC) (40306ES20) and Hieff qPCR SYBR Green Mix (11201ES03) were purchased from YEASEN (China). .. FreeZol Reagent (R711-01) and Hiscript III Reverse Transcriptase (R30201) were purchased from Vazyme Biotech (China). .. Anti-VEGFA antibody was purchased from Abcam (UK).

    Article Title: ZmSKIP enhances drought tolerance by reducing stomatal aperture in maize.
    Article Snippet: To analyze the expression patterns of ZmSKIP in different tissues, total RNA was extracted from different samples using the RNAprep Pure Polysaccharide Polyphenol Plant Total RNA Extraction Kit (TIANGEN, Beijing, China) according to the manufacturer’s instructions. .. Approximately 1–2 μg of total RNA was reverse transcribed into cDNA using Hiscript III Reverse Transcriptase (Vazyme, Nanjing, China) according to the manufacturer’s instructions. ..

    Article Title: The transcriptional cascade CiERF023 <i>-</i> CiERF041 of <i>Citrus ichangenesis</i> regulates small heat shock protein gene <i>CiHSP26.5</i> to modulate cold tolerance
    Article Snippet: .. 543 544 RNA extraction and reverse transcription quantitative PCR (RT–qPCR) analysis 545 To investigate gene expression, total RNA was extracted using a commercial RNA extraction 546 kit (RN33; Aidlab Biotech Co. Ltd, Beijing, China), followed by complementary DNA (cDNA) 547 synthesis with HiScript III Reverse Transcriptase (R323, Vazyme, Nanjing, China) according 548 to the manufacturer's instructions. .. RT-qPCR was performed using ChamQ Universal SYBR 549 qPCR Master Mix (Q711, Vazyme, Nanjing, China) on an ABI 7500 system (Applied 550 Biosystems, Foster City, CA, USA).

    Article Title: Integrated transcriptomics, proteomics, and metabolomics analysis reveals newcastle disease virus reshapes glycerophospholipid metabolism.
    Article Snippet: .. In brief, total RNA was extracted using TRIzol Reagent (Invitrogen, Carlsbad, CA, USA) according to the manufacturer’s instructions. cDNA was reverse transcribed from total RNA using Hiscript III Reverse Transcriptase (Vazyme Biotech, Nanjing, China) and oligo-dT primers. .. RT-qPCR was performed by adding cDNA, primers, and AceQ qPCR SYBR Green Master Mix (Vazyme Biotech).

    Article Title: ZmSKIP enhances drought tolerance by reducing stomatal aperture in maize
    Article Snippet: To analyze the expression patterns of ZmSKIP in different tissues, total RNA was extracted from different samples using the RNAprep Pure Polysaccharide Polyphenol Plant Total RNA Extraction Kit (TIANGEN, Beijing, China) according to the manufacturer’s instructions. .. Approximately 1–2 μg of total RNA was reverse transcribed into cDNA using Hiscript III Reverse Transcriptase (Vazyme, Nanjing, China) according to the manufacturer’s instructions. ..

    Article Title: Knockdown of DVL2 inhibits vascular smooth muscle cell proliferation and migration.
    Article Snippet: .. Total RNA was extracted using RNA-Solv Reagent (Omega Bio-tek, R6830-02) and reverse transcribed into cDNA with HiScript III Reverse Transcriptase (Vazyme, R323-01). qPCR was performed using 10 μL 2× Taq Pro Universal SYBR qPCR Master Mix (Vazyme, Q712-02), 2 μL cDNA, 0.8 μL primers, and 7.2 μL ddH2O in a 20 μL reaction volume. ..

    Article Title: HE4 drives PD-L1 expression in myeloid cells via IFN-γR-JAK-STAT3 signaling to promote tumor immune evasion
    Article Snippet: Total RNA Kit I (D6915-04, Omega Biotek, Norcross, Georgia, USA) according to the manufacturer’s protocol. .. Equal amounts of RNA were reverse transcribed into cDNA via Hiscript III reverse transcriptase (R302-01, Vazyme, Naning, China). .. The resulting cDNA was subjected to real-time PCR using SYBR Green qPCR master mix (Q311, Vazyme).

    Article Title: Spatial multiomics profiling reveals ZFP36-mediated immunometabolic reprogramming in bladder cancer.
    Article Snippet: Bladder cancer remains a significant therapeutic challenge due to its marked heterogeneity and capacity for immune evasion.. Here, we employ spatial metabolomics and spatial transcriptomics to systematically characterize and visualize the metabolic and transcriptional landscapes of bladder cancer.. Our findings identify distinct metabolic and transcriptional profiles across different tumor regions, highlighting heterogeneity and immuneassociated metabolic reprogramming in BLCA.

    RNA Extraction:

    Article Title: The transcriptional cascade CiERF023 <i>-</i> CiERF041 of <i>Citrus ichangenesis</i> regulates small heat shock protein gene <i>CiHSP26.5</i> to modulate cold tolerance
    Article Snippet: .. 543 544 RNA extraction and reverse transcription quantitative PCR (RT–qPCR) analysis 545 To investigate gene expression, total RNA was extracted using a commercial RNA extraction 546 kit (RN33; Aidlab Biotech Co. Ltd, Beijing, China), followed by complementary DNA (cDNA) 547 synthesis with HiScript III Reverse Transcriptase (R323, Vazyme, Nanjing, China) according 548 to the manufacturer's instructions. .. RT-qPCR was performed using ChamQ Universal SYBR 549 qPCR Master Mix (Q711, Vazyme, Nanjing, China) on an ABI 7500 system (Applied 550 Biosystems, Foster City, CA, USA).

    Real-time Polymerase Chain Reaction:

    Article Title: The transcriptional cascade CiERF023 <i>-</i> CiERF041 of <i>Citrus ichangenesis</i> regulates small heat shock protein gene <i>CiHSP26.5</i> to modulate cold tolerance
    Article Snippet: .. 543 544 RNA extraction and reverse transcription quantitative PCR (RT–qPCR) analysis 545 To investigate gene expression, total RNA was extracted using a commercial RNA extraction 546 kit (RN33; Aidlab Biotech Co. Ltd, Beijing, China), followed by complementary DNA (cDNA) 547 synthesis with HiScript III Reverse Transcriptase (R323, Vazyme, Nanjing, China) according 548 to the manufacturer's instructions. .. RT-qPCR was performed using ChamQ Universal SYBR 549 qPCR Master Mix (Q711, Vazyme, Nanjing, China) on an ABI 7500 system (Applied 550 Biosystems, Foster City, CA, USA).

    Article Title: Knockdown of DVL2 inhibits vascular smooth muscle cell proliferation and migration.
    Article Snippet: .. Total RNA was extracted using RNA-Solv Reagent (Omega Bio-tek, R6830-02) and reverse transcribed into cDNA with HiScript III Reverse Transcriptase (Vazyme, R323-01). qPCR was performed using 10 μL 2× Taq Pro Universal SYBR qPCR Master Mix (Vazyme, Q712-02), 2 μL cDNA, 0.8 μL primers, and 7.2 μL ddH2O in a 20 μL reaction volume. ..

    Gene Expression:

    Article Title: The transcriptional cascade CiERF023 <i>-</i> CiERF041 of <i>Citrus ichangenesis</i> regulates small heat shock protein gene <i>CiHSP26.5</i> to modulate cold tolerance
    Article Snippet: .. 543 544 RNA extraction and reverse transcription quantitative PCR (RT–qPCR) analysis 545 To investigate gene expression, total RNA was extracted using a commercial RNA extraction 546 kit (RN33; Aidlab Biotech Co. Ltd, Beijing, China), followed by complementary DNA (cDNA) 547 synthesis with HiScript III Reverse Transcriptase (R323, Vazyme, Nanjing, China) according 548 to the manufacturer's instructions. .. RT-qPCR was performed using ChamQ Universal SYBR 549 qPCR Master Mix (Q711, Vazyme, Nanjing, China) on an ABI 7500 system (Applied 550 Biosystems, Foster City, CA, USA).

    Isolation:

    Article Title: Spatial multiomics profiling reveals ZFP36-mediated immunometabolic reprogramming in bladder cancer.
    Article Snippet: Bladder cancer remains a significant therapeutic challenge due to its marked heterogeneity and capacity for immune evasion.. Here, we employ spatial metabolomics and spatial transcriptomics to systematically characterize and visualize the metabolic and transcriptional landscapes of bladder cancer.. Our findings identify distinct metabolic and transcriptional profiles across different tumor regions, highlighting heterogeneity and immuneassociated metabolic reprogramming in BLCA.

    Synthesized:

    Article Title: Spatial multiomics profiling reveals ZFP36-mediated immunometabolic reprogramming in bladder cancer.
    Article Snippet: Bladder cancer remains a significant therapeutic challenge due to its marked heterogeneity and capacity for immune evasion.. Here, we employ spatial metabolomics and spatial transcriptomics to systematically characterize and visualize the metabolic and transcriptional landscapes of bladder cancer.. Our findings identify distinct metabolic and transcriptional profiles across different tumor regions, highlighting heterogeneity and immuneassociated metabolic reprogramming in BLCA.



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