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hiscript reverse transcriptase  (Vazyme Biotech Co)


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    Structured Review

    Vazyme Biotech Co hiscript reverse transcriptase
    Hiscript Reverse Transcriptase, supplied by Vazyme Biotech Co, used in various techniques. Bioz Stars score: 96/100, based on 726 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/hiscript+iii+reverse+transcriptase/Hiscript+III+Reverse+Transcriptase/pm42843236-157-7-10
    Average 96 stars, based on 726 article reviews
    hiscript reverse transcriptase - by Bioz Stars, 2026-10
    96/100 stars

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    Related Articles

    Reverse Transcription:

    Article Title: Combating VEGFA-siRNA-Induced Metabolic Reprogramming via Glucose Utilization Deprivation.
    Article Snippet: YeaRed Nucleic Acid Gel Stain (10202ES76), Annexin V-FITC/PI Apoptosis Assay Kit (40302ES60), TUNEL Apoptosis Detection Kit (FITC) (40306ES20) and Hieff qPCR SYBR Green Mix (11201ES03) were purchased from YEASEN (China). .. FreeZol Reagent (R711-01) and Hiscript III Reverse Transcriptase (R30201) were purchased from Vazyme Biotech (China). .. Anti-VEGFA antibody was purchased from Abcam (UK).

    Article Title: ZmSKIP enhances drought tolerance by reducing stomatal aperture in maize.
    Article Snippet: To analyze the expression patterns of ZmSKIP in different tissues, total RNA was extracted from different samples using the RNAprep Pure Polysaccharide Polyphenol Plant Total RNA Extraction Kit (TIANGEN, Beijing, China) according to the manufacturer’s instructions. .. Approximately 1–2 μg of total RNA was reverse transcribed into cDNA using Hiscript III Reverse Transcriptase (Vazyme, Nanjing, China) according to the manufacturer’s instructions. ..

    Article Title: ZmSKIP enhances drought tolerance by reducing stomatal aperture in maize
    Article Snippet: To analyze the expression patterns of ZmSKIP in different tissues, total RNA was extracted from different samples using the RNAprep Pure Polysaccharide Polyphenol Plant Total RNA Extraction Kit (TIANGEN, Beijing, China) according to the manufacturer’s instructions. .. Approximately 1–2 μg of total RNA was reverse transcribed into cDNA using Hiscript III Reverse Transcriptase (Vazyme, Nanjing, China) according to the manufacturer’s instructions. ..

    Article Title: Knockdown of DVL2 inhibits vascular smooth muscle cell proliferation and migration.
    Article Snippet: .. Total RNA was extracted using RNA-Solv Reagent (Omega Bio-tek, R6830-02) and reverse transcribed into cDNA with HiScript III Reverse Transcriptase (Vazyme, R323-01). qPCR was performed using 10 μL 2× Taq Pro Universal SYBR qPCR Master Mix (Vazyme, Q712-02), 2 μL cDNA, 0.8 μL primers, and 7.2 μL ddH2O in a 20 μL reaction volume. ..

    Article Title: Avobenzone induces intestinal injury by targeting ATG14 and impairing mitophagy
    Article Snippet: .. The RNA was then reverse transcribed into cDNA using Hiscript III Reverse Transcriptase (Vazyme). .. Subsequently, qRT-PCR was performed to detect the relative expression of the target gene using ChamQ Blue Universal SYBR qPCR Master Mix (Vazyme).

    Article Title: Multi-genome comparative analysis and VIGS validation suggest that GhMAPKKK5 negatively regulates fruiting branch angle in upland cotton
    Article Snippet: Total RNA from Jimian8 plants was isolated using the FastPure Plant Total RNA Isolation Kit (Vazyme, China). .. First-strand cDNA was then prepared with HiScript III Reverse Transcriptase (Vazyme). ..

    Article Title: HE4 drives PD-L1 expression in myeloid cells via IFN-γR-JAK-STAT3 signaling to promote tumor immune evasion
    Article Snippet: Total RNA Kit I (D6915-04, Omega Biotek, Norcross, Georgia, USA) according to the manufacturer’s protocol. .. Equal amounts of RNA were reverse transcribed into cDNA via Hiscript III reverse transcriptase (R302-01, Vazyme, Naning, China). .. The resulting cDNA was subjected to real-time PCR using SYBR Green qPCR master mix (Q311, Vazyme).

    Article Title: Spatial multiomics profiling reveals ZFP36-mediated immunometabolic reprogramming in bladder cancer.
    Article Snippet: Bladder cancer remains a significant therapeutic challenge due to its marked heterogeneity and capacity for immune evasion.. Here, we employ spatial metabolomics and spatial transcriptomics to systematically characterize and visualize the metabolic and transcriptional landscapes of bladder cancer.. Our findings identify distinct metabolic and transcriptional profiles across different tumor regions, highlighting heterogeneity and immuneassociated metabolic reprogramming in BLCA.

    Real-time Polymerase Chain Reaction:

    Article Title: Knockdown of DVL2 inhibits vascular smooth muscle cell proliferation and migration.
    Article Snippet: .. Total RNA was extracted using RNA-Solv Reagent (Omega Bio-tek, R6830-02) and reverse transcribed into cDNA with HiScript III Reverse Transcriptase (Vazyme, R323-01). qPCR was performed using 10 μL 2× Taq Pro Universal SYBR qPCR Master Mix (Vazyme, Q712-02), 2 μL cDNA, 0.8 μL primers, and 7.2 μL ddH2O in a 20 μL reaction volume. ..

    Isolation:

    Article Title: Spatial multiomics profiling reveals ZFP36-mediated immunometabolic reprogramming in bladder cancer.
    Article Snippet: Bladder cancer remains a significant therapeutic challenge due to its marked heterogeneity and capacity for immune evasion.. Here, we employ spatial metabolomics and spatial transcriptomics to systematically characterize and visualize the metabolic and transcriptional landscapes of bladder cancer.. Our findings identify distinct metabolic and transcriptional profiles across different tumor regions, highlighting heterogeneity and immuneassociated metabolic reprogramming in BLCA.

    Synthesized:

    Article Title: Spatial multiomics profiling reveals ZFP36-mediated immunometabolic reprogramming in bladder cancer.
    Article Snippet: Bladder cancer remains a significant therapeutic challenge due to its marked heterogeneity and capacity for immune evasion.. Here, we employ spatial metabolomics and spatial transcriptomics to systematically characterize and visualize the metabolic and transcriptional landscapes of bladder cancer.. Our findings identify distinct metabolic and transcriptional profiles across different tumor regions, highlighting heterogeneity and immuneassociated metabolic reprogramming in BLCA.



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